Nomura Chemical Co., Ltd. Japan • Akira Analytical Solutions Pvt. Ltd. India
Develosil Applications Guide
Practical phase-selection guidance for Develosil ODS-UG, ODS-HG and ODS-MG HPLC columns used in reversed-phase analytical, semi-preparative and preparative workflows.
Nomura Chemical Co., Ltd.
15, Hinode-cho, Seto, Aichi 489-0004, Japan
Tel: +81-561-48-1853
Fax: +81-561-48-1434
Email: info@develosil.net
Akira Analytical Solutions Pvt. Ltd.
MIG-1, Plot No. 02, 9th Phase, KPHB Colony,
Kukatpally, Hyderabad – 500085, Telangana, India
Mobile: +91 93918 52431
Email: sales@akiralabs.com
Website: https://akiralabs.com/
How to Choose a Develosil ODS Column
Develosil ODS-UG, ODS-HG and ODS-MG are reversed-phase ODS/C18 columns for laboratories that need reliable method development, quality-control testing, method transfer and scale-up options. The best choice depends on the validated method, USP phase requirement, analyte hydrophobicity, pH and buffer conditions, sample load, pressure limit and required resolution.
Phase Selection at a Glance
ODS-UG
Use as a strong general-purpose C18 starting point for reversed-phase method development, routine QC, transfer work and workflows that require many available dimensions from semi-micro to preparative scale.
Open ODS-UG specifications →ODS-HG
Use when a high-grade ODS phase is preferred for consistency, peak shape and reproducibility in regulated analytical methods, stability studies and method verification work.
Open ODS-HG specifications →ODS-MG
Use for dependable routine C18 separations where a medium-grade phase, broad analytical dimensions and practical QC compatibility are required.
Open ODS-MG specifications →Selection by Analyte and Method Need
| Requirement | Recommended approach | Useful page |
|---|---|---|
| General hydrophobic small molecules | Start with ODS-UG or the ODS series specified by the validated method. | ODS-UG Columns |
| Regulated QC or transfer work | Match particle size, internal diameter and length first; then confirm system suitability on the chosen UG, HG or MG phase. | USP Listing |
| Need higher efficiency | Consider 3 µm options if instrument pressure and method conditions allow. | ODS-HG Columns |
| Routine robust QC | 5 µm analytical formats are commonly practical for established assays. | ODS-MG Columns |
| Scale-up or purification | Use semi-preparative or preparative dimensions listed in the relevant product family. | Request selection support |
Particle Size and Dimension Guide
3 µm columns are useful when higher efficiency, sharper peaks and improved resolution are required. 5 µm columns are widely used for routine methods and robustness. 10 µm columns are useful where listed for preparative work, lower pressure requirements or scale-up methods.
2.0 mm ID supports semi-micro, lower-solvent workflows. 3.0–4.6 mm ID supports common analytical HPLC methods. 8.0 mm, 10.0 mm, 20.0 mm, 28.0 mm and 50.0 mm ID formats support semi-preparative and preparative applications where listed.
Method Transfer Checklist
For successful method transfer, match the stationary phase, USP phase category, particle size, internal diameter, column length, mobile phase composition, buffer pH, flow rate, temperature, detector settings and sample load as closely as possible. After installation, verify system suitability parameters such as retention time, resolution, tailing factor, theoretical plates and repeatability.
Check USP ListingAsk for SupportFrequently Asked Questions
Which Develosil ODS phase should be selected first for a new reversed-phase method?
For a general C18 reversed-phase starting point, ODS-UG is a practical first screening phase because it is listed in multiple particle sizes and dimensions and supports routine analytical to preparative method development.
When should ODS-HG be considered?
ODS-HG can be considered when the method needs a high-grade ODS phase with reliable peak shape and reproducibility for regulated QC or transfer work.
When is ODS-MG useful?
ODS-MG is useful for routine reversed-phase separations where a dependable medium-grade ODS/C18 phase is suitable for the validated method and system suitability requirements.
Are UG, HG and MG suitable for USP L1 methods?
ODS-UG, ODS-HG and ODS-MG are ODS/C18 reversed-phase chemistries commonly aligned with USP L1-type methods, but final suitability must be confirmed against the exact monograph or validated method.
How do I choose particle size?
Use smaller particles such as 3 µm for higher efficiency and sharper peaks when system pressure allows. Use 5 µm for robust routine QC methods and 10 µm for preparative or lower-pressure applications where listed.
How do I choose column internal diameter?
Use 2.0 mm for semi-micro/solvent-saving work, 3.0 to 4.6 mm for common analytical HPLC methods, and larger internal diameters such as 8.0 mm, 10.0 mm, 20.0 mm, 28.0 mm or 50.0 mm for semi-preparative or preparative scale where listed.
Can Develosil columns be used for method transfer?
Yes, the tables help identify matching phase, particle size, internal diameter and length. Final transfer must be verified by system suitability, resolution, tailing factor, theoretical plates and laboratory SOP acceptance limits.
What information should be shared for column recommendation?
Share the current method, analyte type, USP phase, column dimension, particle size, mobile phase, pH, detector, flow rate, sample load, current problem and required part number if available.